姓 名：毛思锋
职称职务：教授、博导、 苏州市创新创业领军人才（重点资助）
电子邮箱：sfmao@suda.edu.cn
办公地址：国际创新药学院1103-1室
个人简介：
毛思锋，国际创新药学院教授，博士生导师，课题组长（PI）。苏州市创新创业领军人才入选者、中国分析测试协会微纳流控分析委员会委员（2025–2030）。本科及硕士毕业于清华大学，博士毕业于日本东京都立大学。2016年至2020年在清华大学化学系从事博士后工作，随后于2020-2024年在日本东京都立大学任助理教授。2022年被授予东京都立大学“青年领袖科学家”荣誉。
在Chem. Soc. Rev. (IF 40.4), Angew. Chem. (IF 16.1), Adv. Sci. (IF 14.3), Trends Anal. Chem. (IF 11.8), Biosens. Bioelectron. (IF 10.7), Chem. Sci. (IF 7.5), Anal. Chem. (IF 6.8)等国际学术期刊上发表SCI论文 70 余篇，参与撰写专著 2 部。论文被引超过2000次，H因子28。获授权中国专利 10 项、日本专利 1 项。主持及参与国家自然科学基金、日本学术振兴会等国家级项目 6 项，北京市自然科学基金青年基金项目 1 项。担任Journal of Analysis Testing期刊、《分析试验室》期刊青年编委，受邀作为Adv. Sci., Chem. Sci., Trends Anal. Chem., Anal. Chem., Talanta等多种国际学术期刊独立审稿人。
研究方向：
1. 单细胞药物分析与精准医疗
2. 外泌体介导的细胞治疗与机制研究
3. 高灵敏生物传感与疾病标志物检测
4. 器官芯片与药物评价
代表性论文：(†共同第一作者；*通讯作者)
1. X. Hu, H. Luo, N. Kasai*, H. Luo, H. Nakajima, S. Kato, K. Uchiyama, S. Mao*, An inclined push−pull probe for in situ cell staining and calcium channel activation, Anal. Chem. 2025, 96, 5376–5383.
2. C. H. Peng, N. Kasai*, H. Luo, H. Nakajima, S. Kato, K. Uchiyama, S. Mao*, Sustained Synthesis of Ultra-Long Gold Wires via an Open Microfluidic System, ChemPhysChem, 2025, 26, 202500131.
3. H. Luo, N. Kasai, K. Tsumoto, C. Peng, H. Nakajima, S. Kato, H. Zeng*, K. Uchiyama, S. Mao*, Region-specific control of lipid membrane fusion using an open-space fluidic online mixing system, Sensors And Actuators B-Chemical, 2025, 430, 137323.
4. A. Fu, S. Mao*, N. Kasai, H. Zhu, H. Zeng*, Biosensors and Bioelectronics,Dynamic tissue model in vitro and its application for assessment of microplastics-induced toxicity to air-blood barrier (ABB), 2024, 115858.
5. J. Yang, Y. He, Z. Li, X. Yang, Y. Gao, M. Chen, Y. Zheng, S. Mao*, X. Shi*,Intelligent wound dressing for simultaneous in situ detection and elimination of pathogenic bacteria, Acta Biomaterialia, 2024, 174, 177-190.
6. S. Mao*,Recent advances in nanowire sensor assembly using laminar flow in open space,TRAC Trends in Analytical Chemistry, 2023, 159, 116918.
7. H. Lin, N. Kasai, N. Xu, H. Nakajima, S. Kato, H. Zeng, J.-M. Lin*, S. Mao*, and K. Uchiyama, Localized hydrodynamic flow confinement assisted nanowire sensor for ultrasensitive protein detection, Biosensors and Bioelectronics, 2022, 218, 114788.
8. S. Mao*, X. H. Hu, Y. Tanaka, L. Zhou, C. H. Peng, N. Kasai, H. Nakajima, S. Kato, and K. Uchiyama, A chemo-mechanical switchable valve on microfluidic chip based on a thermally responsive block copolymer, Chinese Chemical Letters, 2022, 33(6), 3083-3086.
9. Y. Nishitani, N. Kasai, H. Nakajima, S. Kato, S. Mao*, and K. Uchiyama,Regioselective fabrication of gold nanowires using open-space laminar flow for attomolar protein detection,Chemical Communications, 2022, 58(27), 4308-4311.
10. L. Zhou, N. Kasai, H. Nakajima, S. Kato, S. Mao*, and K. Uchiyama, In Situ Single-Cell Stimulation and Real-Time Electrochemical Detection of Lactate Response Using a Microfluidic Probe, Analytical Chemistry, 2021, 93(24), 8680-8686.
11. N. Xu, H. F. Lin, S. Lin, W. L. Zhang, S. Han, H. Nakajima, S. Mao*, and J. -M. Lin*,A Fluidic Isolation-Assisted Homogeneous-Flow-Pressure Chip-Solid Phase Extraction-Mass Spectrometry System for Online Dynamic Monitoring of 25-Hydroxyvitamin D3 Biotransformation in Cells,Analytical Chemistry, 2021, 93(4), 2273-2280.
12. Q. S. Huang†, S. Mao†, M. Khan, W. W. Li, Q. Zhang, and J.-M. Lin,Single-cell identification by microfluidic-based in situ extracting and online mass spectrometric analysis of phospholipids expression,Chemical Science, 2020, 11(1), 253-256.
13. S. Feng†, S. Mao†, J. X. Dou, W. W. Li, H. F. Li, and J. M. Lin,An open-space microfluidic chip with fluid walls for online detection of VEGF via rolling circle amplification,Chemical Science, 2019, 10(37), 8571-8576.
14. S. Mao, Q. Zhang, W. Liu, Q. S. Huang, M. Khan, W. L. Zhang, C. H. Lin, K. Uchiyama, and J. M. Lin,Chemical operations on a living single cell by open microfluidics for wound repair studies and organelle transport analysis,Chemical Science, 2019, 10(7), 2081-2087.
15. S. Feng†, S. Mao†, Q. Zhang, W. W. Li, and J. M. Lin,Online Analysis of Drug Toxicity to Cells with Shear Stress on an Integrated Microfluidic Chip,ACS Sensors, 2019, 4(2), 521-527.
16. W. Li†, S. Mao†, M. Khan, Q. Zhang, Q. S. Huang, S. Feng, and J. M. Lin,Responses of cellular adhesion strength and stiffness to fluid shear stress during tumor cell rolling motion,ACS Sensors, 2019, 4(6), 1710-1715.
17. S. Mao, W. L. Zhang, Q. S. Huang, M. Khan, H. Li, K. Uchiyama, and J.-M. Lin,In Situ Scatheless Cell Detachment Reveals Correlation between Adhesion Strength and Viability at Single-Cell Resolution,Angewandte Chemie-International Edition, 2018, 57(1), 236-240.
18. S. Mao, Q. Zhang, H. F. Li, W. L. Zhang, Q. S. Huang, M. Khan, and J. M. Lin,Adhesion analysis of single circulating tumor cell on base layer of endothelial cells using open microfluidics,Chemical Science, 2018, 9(39), 7694-7699.
19. Y. Zhang, S. Mao*, Y. Suzuki, Y. Tanaka, M. Kawaguchi, W. F. Zhang, H. L. E. Zeng, H. Nakajima, M. Yang, and K. Uchiyama*, Elaborately programmed nanowires fabricated using a tapered push-pull nozzle system, Chemical Communications, 2018, 54(7), 719-722.
20. S. Mao, Y. Zhang, Q. Zhang, J. M. Lin, and K. Uchiyama,Local surface modification at precise position using a chemical pen,Talanta, 2018, 187, 246-251.
21. S. Mao, Q. Zhang, H. F. Li, Q. S. Huang, M. Khan, K. Uchiyama, and J. M. Lin,Measurement of Cell-Matrix Adhesion at Single-Cell Resolution Reveals the Functions of Biomaterials for Adherent Cell Culture,Analytical Chemistry, 2018, 90(15), 9637-9643.
22. S. Mao, W. W. Li, Q. Zhang, W. L. Zhang, Q. S. Huang, and J. M. Lin,Cell analysis on chip-mass spectrometry,TRAC-Trends in Analytical Chemistry, 2018, 107, 43-59.
23. C. H. Lin†, L. Lin†, S. Mao†, L. J. Yang, L. L. Yi, X. X. Lin, J. M. Wang, Z. X. Lin, and J. M. Lin,Reconstituting Glioma Perivascular Niches on a Chip for Insights into Chemoresistance of Glioma,Analytical Chemistry, 2018, 90(17), 10326-10333.
24. S. Mao, Y. Zhang, H. F. Li, H. L. Zeng, J. M. Lin, and K. Uchiyama,Writing of nanowires via high viscosity-induced nano diffusive layer,Journal of Materials Chemistry C, 2017, 5(45), 11666-11671.
25. S. Mao, Y. Zhang, W. Zhang, H. Zeng, H. Nakajima, J.-M. Lin, and Katsumi Uchiyama,Convection-Diffusion Layer in an "Open Space" for Local Surface Treatment and Microfabrication using a Four-Aperture Microchemical Pen,Chemphyschem, 2017, 18, 2357-2363.
26. S. Mao, C. Sato, Y. Suzuki, J. Yang, H. Zeng, H. Nakajima, M. Yang, J.-M. Lin, and K. Uchiyama,Microchemical Pen: An Open Microreactor for Region-Selective Surface Modification,Chemphyschem, 2016, 17(20), 3155-3159.
27. N. Wang†, S. Mao†, W. Liu, J. Wu, H. Li, and J.-M. Lin,Online monodisperse droplets based liquid-liquid extraction on a continuously flowing system by using microfluidic devices,RSC Advances, 2014, 4(23), 11919-11926.
28. S. Mao, J. Zhang, H. Li, and J.-M. Lin,Strategy for Signaling Molecule Detection by Using an Integrated Microfluidic Device Coupled with Mass Spectrometry to Study Cell-to-Cell Communication,Analytical Chemistry, 2013, 85(2), 868-876.
29. S. Mao, D. Gao, W. Liu, H. Wei, and J.-M. Lin,Imitation of drug metabolism in human liver and cytotoxicity assay using a microfluidic device coupled to mass spectrometric detection,Lab on a Chip, 2012, 12(1), 219-226.
30. H. Lin, N. Xu, S. Mao, H. Nakajima, J.-M. Lin, K. Uchiyama, N. Kasai, L. Lin, Robust Long-Nanowire Fabrication by Clean-Phase-Assisted Micro Chemical Pen for Enhanced Bioassay Sensitivity, Analytical Chemistry, 2024, 96. 14339-14347.
31. Q. Zhang, S. Feng, L. Lin, S. Mao, and J.- M. Lin,Emerging open microfluidics for cell manipulation,Chemical Society Reviews, 2021, 50(9), 5333-5348.
32. N. Wu, Y. J. Zheng, L. Lin, S. Mao, Z. H. Li, and J. -M. Lin,Controllable synthesis of multicompartmental particles using 3D microfluidics,Angewandte Chemie-International Edition, 2020, 59(6), 2225-2229.
33. J. T. Chen, S. Mao, Z. Y. He, L. J. Yang, J. F. Zhang, J. M. Lin, and Z. X. Lin,Proteomic distributions in CD34+ microvascular niche patterns of glioblastoma,Journal of Histochemistry & Cytochemistry, 2022, 70(1), 99-110.
34. Q. Zhang, S. Mao, W. W. Li, Q. S. Huang, S. Feng, Z. Y. Hong, and J. -M. Lin,Microfluidic adhesion analysis of single glioma cells for evaluating the effect of drugs,Science China-Chemistry, 2020, 63(6), 865-870.
35. T. Xie, N. Li, S. Mao, Q. Zhang, and J. -M. Lin,Cell Heterogeneity Revealed by On-Chip Angiogenic Endothelial Cell Migration, ACS Omega, 2020, 5(8), 3857-3862.
36. J. Dou, S. Mao, H. F. Li, and J. M. Lin,Combination stiffness gradient with chemical stimulation directs glioma cell migration on a microfluidic chip,Analytical Chemistry, 2020, 92(1), 892-898.
37. Z. Zhong, S. Mao, H. F. Lin, H. F. Li, J. H. Lin, and J. M. Lin,Alteration of intracellular metabolome in osteosarcoma stem cells revealed by liquid chromatography-tandem mass spectrometry,Talanta, 2019, 204, 6-12.
38. Y. Zheng, Z. N. Wu, M. Khan, S. Mao, K. Manibalan, N. Li, J. M. Lin, and L. Lin,Multifunctional regulation of 3D cell-laden microsphere culture on an integrated microfluidic device,Analytical Chemistry, 2019, 91(19), 12283-12289.
39. W. Zhang, S. Mao, Z. Y. He, Z. N. Wu, and J. M. Lin,In situ monitoring of fluid shear stress enhanced adherence of bacteria to cancer cells on microfluidic chip,Analytical Chemistry, 2019, 91(9), 5973-5979.
40. Q. Zhang, S. Mao, M. Khan, S. Feng, W. L. Zhang, W. W. Li, and J. M. Lin,In situ partial treatment of single cells by laminar flow in the “open space”,Analytical Chemistry, 2019, 91(2), 1644-1650.
41. S. Wang, S. Mao, M. Li, H. F. Li, and J. M. Lin,Near-physiological microenvironment simulation on chip to evaluate drug resistance of different loci in tumour mass,Talanta, 2019, 191, 67-73.
42. H. Lin, S. Mao, H. Zeng, Y. Zhang, M. Kawaguchi, Y. Tanaka, J. M. Lin, and K. Uchiyama,Selective fabrication of nanowires with high aspect ratios using a diffusion mixing reaction system for applications in temperature sensing,Analytical Chemistry, 2019, 91(11), 7346-7352.
43. W. Li, M. Khan, H. F. Li, L. Lin, S. Mao, and J. M. Lin,Homogenous deposition of matrix-analyte cocrystals on gold-nanobowl arrays for improving MALDI-MS signal reproducibility,Chemical Communications, 2019, 55(15), 2166-2169.
44. M. Li, S. Mao, S. Q. Wang, H. F. Li, and J. M. Lin,Chip-based SALDI-MS for rapid determination of intracellular ratios of glutathione to glutathione disulfide,Science China-Chemistry, 2019, 62(1), 142-150.
45. M. Khan, W. W. Li, S. Mao, S. N. A. Shah, and J. M. Lin,Real-Time Imaging of Ammonia Release from Single Live Cells via Liquid Crystal Droplets Immobilized on the Cell Membrane,Advanced Science, 2019, 6(20).
46. Q. Huang, S. Mao, M. Khan, and J. M. Lin,Single-cell assay on microfluidic devices,Analyst, 2019, 144(3), 808-823.
47. L. Zhou, S. Mao, Q. S. Huang, X. W. He, and J. M. Lin,Inhibition of anaerobic probiotics on colorectal cancer cells using intestinal microfluidic systems,Science China-Chemistry, 2018, 61(8), 1034-1042.
48. Z. Zhong, S. Mao, H. F. Lin, J. M. Lin, and J. H. Lin,Comparative proteomics of cancer stem cells in osteosarcoma using ultra-high-performance liquid chromatography and Orbitrap Fusion mass spectrometer,Talanta, 2018, 178, 362-368.
49. Y. Zhang, H. L. Zeng, S. Mao, S. Kondo, H. Nakajima, S. Kato, C. L. Ren, and K. Uchiyama,Reversibly Switching Molecular Spectra,ACS Applied Materials & Interfaces, 2018, 10(27), 23247-23253.
50. W. L. Zhang, Z. Y. He, L. L. Yi, S. Mao, H. F. Li, and J. M. Lin, Biosensors and Bioelectronics,A dual-functional microfluidic chip for on-line detection of interleukin-8 based on rolling circle amplification, 2018, 102, 652-660.
51. Z. N. Wu, L. Lin, M. Khan, W. F. Zhang, S. Mao, Y. J. Zheng, Z. H. Li, and J. M. Lin,DNA-Mediated rolling circle amplification for ultrasensitive detection of thrombin using MALDI-TOF mass spectrometry,Chemical Communications, 2018, 54(82), 11546-11549.
52. Z. N. Wu, M. Khan, S. Mao, L. Lin, and J. M. Lin,Combination of nano-material enrichment and dead-end filtration for uniform and rapid sample preparation in matrix-assisted laser desorption/ionization mass spectrometry,Talanta, 2018, 181, 217-223.
53. J. M. Wang, S. Mao, H. F. Li, and J. M. Lin,Multi-DNAzymes-functionalized gold nanoparticles for ultrasensitive chemiluminescence detection of thrombin on microchip,Analytica Chimica Acta, 2018, 1027, 76-82.
54. W. W. Li, M. Khan, S. Mao, S. Feng, and J. M. Lin,Advances in tumor-endothelial cells co-culture and interaction on microfluidics,Journal of Pharmaceutical Analysis, 2018, 8(4), 210-218.
55. M. Khan, Z. N. Wu, S. Mao, S. N. A. Shah, and J. M. Lin,Controlled grafted poly(quartanized-4-vinylpyridine-co-acrylicacid) brushes allure bacteria for effective antimicrobial surfaces,Journal of Materials Chemistry B, 2018, 6(22), 3782-3791.
56. M. Khan, S. Mao, W. W. Li, and J. M. Lin,Microfluidic devices in the fast-growing domain of single-cell analysis,Chemistry-a European Journal, 2018, 24(58), 15398-15420.
57. Q. S. Huang, S. Mao, M. Khan, L. Zhou, and J. M. Lin,Dean flow assisted cell ordering system for lipid profiling in single-cells using mass spectrometry,Chemical Communications, 2018, 54(21), 2595-2598.
58. Z. Y. He, W. L. Zhang, S. Mao, N. Li, H. F. Li, and J. M. Lin,Shear Stress-Enhanced Internalization of Cell Membrane Proteins Indicated by a Hairpin-Type DNA Probe,Analytical Chemistry, 2018, 90(9), 5540-5545.
59. H. Zeng, Y. Zhang, S. Mao, H. Nakajima, and K. Uchiyama,A reversibly electro-controllable polymer brush for electro-switchable friction,Journal of Materials Chemistry C, 2017, 5(24), 5877-5881.
60. S. Shan, Z. Y. He, S. Mao, M. S. Jie, L. L. Yi, and J. M. Lin,Quantitative determination of VEGF165 in cell culture medium by aptamer sandwich based chemiluminescence assay,Talanta, 2017, 171, 197-203.
61. M. Jie, S. Mao, H. Liu, Z. He, H. F. Li, and J. M. Lin,Evaluation of drug combination for glioblastoma based on an intestine-liver metabolic model on microchip,Analyst, 2017,142 (19), 3629-3638.
62. M. Jie, M. Jie, S. Mao, H. F. Li, and J. M. Lin,Multi-channel microfluidic chip-mass spectrometry platform for cell analysis,Chinese Chemical Letters, 2017,28,1625-1630.
63. J. T. Chen, S. Mao, H. F. Li, M. C. Zheng, L. G. Yi, J. M. Lin, and Z. X. Lin,The pathological structure of the perivascular niche in different microvascular patterns of glioblastoma,PloS One, 2017, 12(8).
64. W. Zhang, S. Mao, J. Yang, H. Zeng, H. Nakajima, S. Kato, and K. Uchiyama,The use of an inkjet injection technique in immunoassays by quantitative on-line electrophoretically mediated microanalysis,Journal of Chromatography A, 2016, 1477, 127-131.
65. J. Yang, D. Katagiri, S. Mao, H. Zeng, H. Nakajima, S. Kato, and K. Uchiyama,Inkjet printing based assembly of thermoresponsive core-shell polymer microcapsules for controlled drug release,Journal of Materials Chemistry B, 2016, 4(23), 4156-4163.
66. J. Yang, D. Katagiri, S. Mao, H. Zeng, H. Nakajima, and K. Uchiyama,Generation of controlled monodisperse porous polymer particles by dipped inkjet injection,RSC Advances, 2015, 5(10), 7297-7303.
67. J. Yang, M. Hida, S. Mao, H. Zeng, H. Nakajima, and K. Uchiyama,A chemo-mechanical switch for controllable water transportation based on a thermally responsive block copolymer,Chemical Communications,2014, 50(71), 10265-10268.
68. W. Liu, S. Mao, J. Wu, and J.-M. Lin,Development and applications of paper-based electrospray ionization-mass spectrometry for monitoring of sequentially generated droplets,Analyst, 2013, 138(7), 2163-2170.
69. F. Chen, S. Mao, H. Zeng, S. Xue, J. Yang, H. Nakajima, J.-M. Lin, and K. Uchiyama,Inkjet nanoinjection for high-throughput chemiluminescence immunoassay on multicapillary glass plate,Analytical Chemistry, 2013, 85(15), 7413-7418.
70. J. Liu, D. Gao, S. Mao, and J.-M. Lin,A microfluidic photolithography for controlled encapsulation of single cells inside hydrogel microstructures,Science China-Chemistry, 2012, 55(4), 494-501.
71. H. Wei, H. Li, S. Mao, and J.-M. Lin,Cell Signaling Analysis by Mass Spectrometry under Coculture Conditions on an Integrated Microfluidic Device,Analytical Chemistry, 2011, 83(24), 9306-9313.
72. W. Liu, H. Wei, Z. Lin, S. Mao, and J.-M. Lin, Biosensors and Bioelectronics,Rare cell chemiluminescence detection based on aptamer-specific capture in microfluidic channels, 2011, 28(1), 438-442.
代表性专著：
1. S. Mao, J.-M. Lin, Chapter 9 Microfluidic Chip-Based Live Single-Cell Probes. Microfluidics for Single-Cell Analysis, Springer, Singapore, 2019.
2. 毛思锋，林金明，第六章开放式微流体细胞分析。微流控细胞分析，北京化工出版社，2021.
招聘信息：
拟招收博士后2-3名、博士研究生1名及硕士研究生若干名。有意者请将个人简历（包括个人基本情况、教育和工作经历、发表论文情况及科研工作概述）发送至sfmao@suda.edu.cn。
欢迎具有药学、化学或生物医学背景的青年报考加入我们！